[AlexoTech] Amyloid-Beta 1-40, 15N Uniform Label (0.2 mg) Human, Recombinant

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AlexoTech의 Amyloid-Beta 제품을 소개드립니다.
 

Description: Recombinant Amyloid-Beta-Peptide (1-40) uniformly ¹⁵N labeled
Article no: ABN-100-02
Amount: 0.2mg
Format: Lyophilized
Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV
Purity: 95% by Chromatography and SDS-PAGE
Counter Ion: Ammonium Acetate
Storage: Store at -20°C upon arrival.
Source: Protein expressed in Escherichia coli using ¹⁵NH₄-Cl as sole nitrogen source.
Solubility:
Alexotech has developed a proprietary technique of preparing the amyloid β-peptide (1-40) having a superior solubility. It is however, of utmost importance to follow our recommendations of solubilisation: To efficiently solubilise the amyloid β-peptide (1-40) the pH should briefly be raised to between 11-12. This can be accomplished by 20 mM of NaOH, however, at high peptide concentrations a higher NaOH concentration may be required due to the intrinsic buffering capacity of the peptide. The pH should therefore always be monitored and if necessary adjusted. After solubilisation the pH can be adjusted using the buffer of choice.
 

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